Surface Antigens on Interspecies Hybrid Cells

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Abstract
Interspecies hybrid cells were obtained by Sendai virus-mediated cell fusion followed by propagation of cells in media exerting proper biochemical pressures. The mixed agglutination test with cell cultures was employed for the analysis of cell surface antigens. Rabbit immune sera were used to identify species-specific antigens. All man-mouse hybrid lines studied contained antigens characteristic for the mouse, and most but not all lines had antigens characteristic for man. Antigens of both parents were shown in man-hamster and man-rat hybrids. Histocompatibility antigens of man-mouse hybrids were detected by human and murine antisera containing proper isoantibodies. Here again murine antigens were found in all lines, whereas human antigens were demonstrated in most but not in all lines. Karyotypic analyses of man-mouse hybrid cells were carried out. From a comparison of the serologic and karyotypic studies, only a tentative conclusion could be drawn that human chromosomes of group C or D may be important for determining human antigens on the cell surface.

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