A cellular protein that competes with SV40 T antigen for binding to the retinoblastoma gene product
- 1 March 1991
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 350 (6314) , 160-162
- https://doi.org/10.1038/350160a0
Abstract
TUMOUR-suppressor genes, such as the human retinoblastoma susceptibility gene (Rb), are widely recognized as being vital in the control of cell growth and tumour formation1. This role is indicated, in part, by the suppression of tumorigenicity of human tumour cells after retrovirus-mediated Rb replacement2–4. How Rb acts to bring about this suppression is not clear5 but one clue is that the Rb protein forms complexes with the transforming oncoproteins of several DNA tumour viruses6–8, and that two regions of Rb essential for such binding frequently contain mutations in tumour cells9,10. These observations suggest that endogenous cellular proteins might exist that bind to the same regions of Rb and thereby mediate its function. We report here the identification of one such human cellular Rb-associated protein of relative molecular mass 46,000 (46K) (RbAP46). Two lines of evidence support the notion that RbAP46 and simian virus 40 T antigen have homologous Rb-binding properties: first, several mutated Rb proteins that failed to bind to T also did not associate with RbAP46; and second, both T antigen and T peptide (amino acids 101–118) were able to compete with RbAP46 for binding to Rb. The apparent targeting of the RbAP46–Rb interaction by oncoproteins of DNA tumour viruses strongly suggests that formation of this complex is functionally important.Keywords
This publication has 9 references indexed in Scilit:
- Negative regulation of human c-fos expression by thes retinoblastoma gene productNature, 1990
- Suppression of Tumorigenicity of Human Prostate Carcinoma Cells by Replacing a Mutated RB GeneScience, 1990
- The product of the retinoblastoma susceptibility gene has properties of a cell cycle regulatory elementCell, 1989
- The Human Papilloma Virus-16 E7 Oncoprotein Is Able to Bind to the Retinoblastoma Gene ProductScience, 1989
- Suppression of the Neoplastic Phenotype by Replacement of the RB Gene in Human Cancer CellsScience, 1988
- Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene productNature, 1988
- SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility geneCell, 1988
- The Approaching Era of the Tumor Suppressor GenesScience, 1987
- Vectors for selective expression of cloned DNAs by T7 RNA polymeraseGene, 1987