Culture of Ethiopian Strains of Borrelia recurrentis

1 January 1973

journal article
research article
Published by American Society for Microbiology in Applied Microbiology

Vol. 25 (6) , 935-939
https://doi.org/10.1128/aem.25.6.935-939.1973

Abstract

A number of standard bacteriological media with supplements were tested for their ability to support in vitro growth of Ethiopian strains of Borrelia recurrentis. Propagation of 18 out of 21 strains occurred in Trypticase soy yeast broth to which bovine albumin (fraction V), N-acetyl glucosamine, and sodium pyruvate had been added. This medium supported a population of 10⁷ organisms per ml and yielded a harvest of four to five times the original inoculum during the logarithmic phase of growth. Maximal yield varied from 1.4 × 10⁷ to 3.4 × 10⁷ organisms per ml. Generation time in optimal media was 11.3 h. Lesser multiplication of organisms occurred in other media tested. Strains from primary cultures were infective for the green monkey. Recovery of viable organisms from subculture has not been successful. Images

Keywords

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