DNA stretching on functionalized gold surfaces
Open Access
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (3) , 492-497
- https://doi.org/10.1093/nar/22.3.492
Abstract
We describe a method for anchoring bacteriophage λ DNA by one end to gold by Au - blotin - streptavidin - blotin - DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned.Keywords
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