Non-Chromosomal Antibiotic Resistance in Bacteria, III: Isolation of the Discrete Transfer Unit of the R-Factor R1
- 1 October 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (2) , 510-516
- https://doi.org/10.1073/pnas.67.2.510
Abstract
A covalently-closed circular DNA species, banding at a buoyant density of rho = 1.709 g/cm(3) in CsCl, has been identified in antibiotic-sensitive colonies of E. coli strain AB2463 (rec A(-)) after mating with a Proteus mirabilis strain that carries the R-factor, R1. This plasmid, which represents a stable segregant of R1 that has lost all of the drug resistance determinants present on the parent R-factor but which has retained its ability to be transferred by conjugation, fulfills the functional definition of the R-factor transfer unit (RTF).Keywords
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