rRNA proceesing: removal of only nineteen bas at the gap between 28Sα and 28Sβ rRNAs inSciara coprophila

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Abstract
We have determined the sequence of the rDNA region between the 28Sα and 28Sβ rRRA coding segments (termed a “gap”) in the insect Sciara corprophila and have used S1 nuclease mapping and cDNA primer extension to define the 5′ and 3′ boundaries of the gap. Only 19 bases found in rDNA at the gap region are absent from mature 28S rRNA. Eukaryotic rRNAs contain stretches of nucleotides (“expansion segments”) which are absent in E. coli rRNA. The gap region in Sciara is located within expansion segment V. Therefore, the excision of 19 bases in the Sciara gap suggests that a large portion of expansion segment V plays no function in mature ribosomes. Specific sequences conserved in Sciara and Drosophila are considered as candidates for recognition signals for the excision of the gap transcript.