THE BIARMED CHROMOSOMES OF MOUSE CELL LINE LMTK–

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Abstract
Staining for fluorescent bands and centromeric heterochromatin showed that normal mouse chromosomes could be identified as specific arms in most of the biarmed chromosomes of the mouse cell line LMTK–. Seven marker chromosomes were also characterized. The karyotype was heterogeneous with an average of 9.4 biarmed chromosomes per cell. Five specific biarmed chromosomes were present in at least 90% of the cells examined. The results indicate the presence of both relatively stable and highly variable components in the in vitro karyotype. Evidence is presented which indicates that the biarmed chromosomes have been formed by reciprocal translocation, centric fusion with retention of both centromeres, and centromeric misdivision with isochromosome formation.