Monoclonal antibodies in studying immunological phenotypes of neoplastic diseases

Publisher Website
Google Scholar
Abstract
Six hybridoma clones were obtained secreting monoclonal antibodies (Mab) against differentiating antigens of human hemopoietic cells. ICO‐1 Mab detect la‐like (Dr) antigens. Mab react with IMymphocytes and monocytes without detecting antigens on granulocytes and T‐cells. Antigen expression was enhanced following cell activation in a blast cell transformation test and mixed lymphocyte culture (MLC). ICO‐1 Mab blocked MLC. The molecular weights of the antigen were 29 and 34 kilodaltons. Comparative studies of ICO‐1 Mab with other Mab against la‐like antigens revealed their identical reactivity. ICO‐11 Mab detect the antigen on 4% of Wood lymphocytes, 75% thymocytes, monoMasts, and CFU‐GM. These Mab block NK‐cell activity of blood mononuclear cells. ICO‐GM‐1 Mab detect the antigen on myelomonocytic cells and their precursors, but not CFU‐GM. These Mab block binding of the C3Bi complement component to CR3 receptor and NK‐cell activity. ICO‐G2 Mab detect the antigen expressed at final stages of granulocyte differentiation. ICO‐10 Mab detect the antigen on early thymocytes and ICO‐02 on undifferentiated blast cells. Mab were shown to be applicable for human leukemia and lymphoma immune diagnosis.

This publication has 15 references indexed in Scilit: