Effect of Artificially Inserted Intron on Gene Expression inSaccharomyces cerevisiae
- 1 January 1994
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA and Cell Biology
- Vol. 13 (1) , 51-58
- https://doi.org/10.1089/dna.1994.13.51
Abstract
The intron of the yeast RP51A gene was cloned with precision using the polymerase chain reaction (PCR) amplification method, and then inserted into several different positions of the yeast URA3 gene as well as the PGK–lacZ fusion gene without introduction of additional exon sequences. Analysis of transcripts of these genes showed that an intron inserted near the transcription start site of the gene was spliced out efficiently, whereas the same intron sequences inserted 200 bp or further downstream of the start site were not, resulting in a reduced level of mRNA. These results explain why intron-containing genes in yeast usually have an intron near the 5′ end.Keywords
This publication has 25 references indexed in Scilit:
- Meiosis-specific RNA splicing in yeastCell, 1991
- Control of Gene Expression by Artificial Introns in Saccharomyces cerevisiaeScience, 1989
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Pseudogenes in yeast?Cell, 1987
- PRE-mRNA SPLICINGAnnual Review of Genetics, 1986
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- mRNA splicing efficiency in yeast and the contribution of nonconserved sequencesCell, 1985
- Structure and function of the yeast URA3 gene: expression in Escherichia coliGene, 1984
- Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeastCell, 1984
- Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicingCell, 1983