Production and Gene Expression of IL-8-Iike Cytokine GRO/CINC-1 in Rat Nasal Mucosa

Publisher Website
Google Scholar
Abstract
Growth-regulated gene product/cytokine-induced neutrophil chemoattractant (GRO/CINQ-1 is a rat chemokine with structural and functional homology to human IL-8. Chemokines are a family of cytokines whose participation in nasal inflammation in vivo remains to be established. Using ELISA and RT-PCR, we investigated the production and gene expression of GRO/CINC-1 in rat nasal lavage and mucosa in vivo. GRO/CINC-1 in nasal lavage was produced by stimulation of LPS, ConA and ILl-beta. GRO/CINC-1 showed time- and dose-dependent production under all stimulants, but was more slowly induced by IL-1 beta. The steady-peak of the GRO/CINC-1 production remained at 3 h with LPS or ConA exposure, whereas it lasted 4 h or more after IL-1 beta exposure. At the time of peak production of GRO/CINC-1, we found that mRNA for the GRO/CINC-1 was induced in the nasal mucosa. The mRNA of the related inflammatory cytokines TNF-alpha and IFN-gamma were also expressed in nasal mucosa with stimulation of these reagents. Thus, this study revealed that exposure to bacterial endotoxin, mitogenic reagent and also IL-1 beta induced the production and gene expression of the neutrophil chemoattractant GRO/CINC-1 in rat nasal mucosa in vivo. This investigation of the characteristics of IL-8 family in nasal mucosa using rat models has extended the functional concept of cytokines in the inflammatory condition of nasal cavity in humans.

This publication has 11 references indexed in Scilit: