In vitro mutagenicity of water contaminants in complex mixtures

Publisher Website
Google Scholar
Abstract
Trihalomethanes, Carbon tetrachloride and trichloroethylene were tested in single, binary and multi‐complex mixtures using standard tester strains TA1535, TA1537, TA98 and TA100 of Salmonella typhimurium with and without addition of an in vitro metabolizing fraction S‐9. Chloroform (CHCl3) was found to be mutagenic in all strains without S‐9 activation. However, when tested with Bromoform (15%), which was nonmutagenic singly, the combined effect of the mixture was nonmutagenic. CCl4 was a direct mutagen (without S‐9) in all strains except TA 1535. When combined with 85% CHCl3, only strains TA1535 and TA1537 were mutagenic. When tested with mammalian activation (S‐9), CCl4 was mutagenic in all strains. However, when tested with CHCl3 (CHCl3 and CCl4 ‐ 85 : 15), the mutagenic capability was lost. With or without S‐9 Activation multi‐complex mixture of CHCl3, CCl4 and TCE (85 : 8 : 7) was mutagenic for a narrow range of doses in all strains.

This publication has 8 references indexed in Scilit: