Role of Interleukin-1β in Activating the CD11chighCD45RB−Dendritic Cell Subset and PrimingLeishmania amazonensis- Specific CD4+T Cells In Vitro and In Vivo

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Abstract
Cutaneous leishmaniasis associated withLeishmania amazonensisinfection is characterized by uncontrolled parasite replication and profound immunosuppression; however, the underlying mechanisms remain largely unclear. One possibility is that theL. amazonensisparasite modulates antigen-presenting cells, favoring the generation of pathogenic Th cells that are capable of recruiting leukocytes but insufficient to fully activate their microbicidal activities. To test this possibility, we infected bone marrow-derived dendritic cells (DCs) of C57BL/6 mice withL. amazonensisorLeishmania majorpromastigotes and assessed the activation of DC subsets and their capacity in priming CD4+T cells in vitro. In comparison toL. majorcontrols,L. amazonensis-infected DCs secreted lower levels of interleukin-1α (IL-1α) and IL-1β, were less potent in activating the IL-12p40-producing CD11chighCD45RB−CD83+CD40+DC subset, and preferentially activated CD4+T cells with a IFN-γlowIL-10highIL-17highphenotype. Although the addition of IL-1β at the time of infection markedly enhanced DC activation and T-cell priming, it did not skew the cytokine profile of DCs and pathogenic Th cells, as local injection of IL-1β followingL. amazonensisinfection accelerated Th cell activation and disease progression. This study suggests that intrinsic defects at the level of DC activation are responsible for the susceptible phenotype inL. amazonensis-infected hosts and that this parasite may have evolved unique mechanisms to interfere with innate and adaptive immunity.