Receptor-Triggered but Alkylation-Arrested Env of Murine Leukemia Virus Reveals the Transmembrane Subunit in a Prehairpin Conformation
- 1 October 2006
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 80 (19) , 9921-9925
- https://doi.org/10.1128/jvi.00380-06
Abstract
A central feature of the prevailing model for retrovirus fusion is conversion of the transmembrane (TM) subunit from a prehairpin to a hairpin-like structure. The fusion inhibition of many retroviruses, except murine leukemia virus (MLV), with peptides corresponding to interacting regions in the hairpin supports the model. MLV fusion is controlled by isomerization of the intersubunit disulfide in Env. We show here that TM peptides bind to MLV Env that has been arrested at an intermediate stage of activation by alkylation of the isomerization-active thiol in the surface subunit. This inhibits fusion rescue by dithiothreitol-mediated reduction of the surface protein-TM disulfide.Keywords
This publication has 35 references indexed in Scilit:
- Kinetic Analyses of the Surface-Transmembrane Disulfide Bond Isomerization-Controlled Fusion Activation Pathway in Moloney Murine Leukemia VirusJournal of Virology, 2005
- Inhibition of Murine Leukemia Virus Envelope Protein (Env) Processing by Intracellular Expression of the Env N-Terminal Heptad Repeat RegionJournal of Virology, 2005
- A Study of the Membrane–Water Interface Region of Membrane ProteinsJournal of Molecular Biology, 2005
- The Cytoplasmic Tail Slows the Folding of Human Immunodeficiency Virus Type 1 Env from a Late Prebundle Configuration into the Six-Helix BundleJournal of Virology, 2005
- Heptad Repeat 2-Based Peptides Inhibit Avian Sarcoma and Leukosis Virus Subgroup A Infection and Identify a Fusion IntermediateJournal of Virology, 2004
- Sequential Roles of Receptor Binding and Low pH in Forming Prehairpin and Hairpin Conformations of a Retroviral Envelope GlycoproteinJournal of Virology, 2004
- The Prefusogenic Intermediate of HIV-1 gp41 Contains Exposed C-peptide RegionsJournal of Biological Chemistry, 2003
- Evidence That the Transition of HIV-1 Gp41 into a Six-Helix Bundle, Not the Bundle Configuration, Induces Membrane FusionThe Journal of cell biology, 2000
- Atomic structure of the ectodomain from HIV-1 gp41Nature, 1997
- Peptides corresponding to a predictive alpha-helical domain of human immunodeficiency virus type 1 gp41 are potent inhibitors of virus infection.Proceedings of the National Academy of Sciences, 1994