Cytological studies of early stages of powdery mildew in barley and wheat. IX. Effect of various inorganic salts on the occurrence of autofluorescence at penetration sites of appressoria of Erysiphe graminis hordei

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Abstract
The effect of varoius inorganic salts on the occurrence of autofluorescence at penetration sites of appressoria of E. graminis f. sp. hordei in barley coleoptiles was examined by fluorescence microscopy. Divalent cations such as Ca2+, Mg2+ and Mn2+ enhanced fluorescence to varying degrees. Among them, Ca2+ had the greatest enhancing effect; intense fluorescence occurred around penetration sites within 10 min after the inoculated coleoptiles were transferred from water to CaCl2 solution. However, other divalent cations such as Zn2+ and Fe2+ showed cytotoxicity to coleoptiles and did not elicit fluorescence. Monovalent cations such as K+, Na+ and Li+ did not show any enhancing effect independently. Anions seemed to be associated with enhancement of the fluorescence in combination with their related divalent cations but not independently. An observed elimination of fluorescence by EDTA suggests that the enhancing effect of some of the divalent cations may be associated with their interaction with a substance(s) which fluoresces only in combination with these cations.