Modulation excitation spectrophotometry

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Abstract
Modulation excitation (ME) spectrophotometry is a technique to measure the absorption spectra of short lived photoproduced transients, such as excited states of molecules, and to measure their lifetimes or kinetics of formation and disappearance. Traditionally such measurements have been made by flash photolysis and similar methods, in which the samples are flashed with an intense pulse of light producing a large instantaneous population of the excited species followed by a second pulse within the lifetime of the species to monitor the absorption of the transient.

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