CHARACTERISTICS OF STREPTOMYCES GRISEUS STRAINS RESISTANT TO PHAGE

Abstract
Resistant strains of Streptomyces griseus were developed which retain indefinitely their resistance toward the phage with which they were developed, but were not resistant to unrelated phages. These strains adsorb the phage to which they are resistant and release it without multiplication. It is postulated, from 3 lines of evidence, that the phage was carried externally on the resistant strains. Release of phage occurs upon aging of the resistant host, or can be made to occur at will by reversal of the primary adsorption step by ionic exchange. Addition of antiserum to a resistant strain with phage adsorbed to that strain inactivates all free phage. Upon removal of the antiserum, the free phage titer immediately begins to increase.
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