Characterization of Monoclonal Antibodies Against Mouse and Rat Platelet Glycoprotein V (CD42d)
- 1 December 2000
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 19 (6) , 455-461
- https://doi.org/10.1089/027245700750053940
Abstract
The mouse- and rat-platelet-specific hamster monoclonal antibody (MAb) 1C2, previously found to react with a thrombin-sensitive 74-kD glycoprotein, was now shown to recognize platelet glycoprotein V (GPV, CD42d). 1C2 reacted with NIH-3T3 cells in which recombinant mouse or rat GPV was expressed. Both 1C2 and 4A5, another mouse-platelet-specific rat MAb, immunoprecipitated GVP, although they recognized different epitopes. Side-by-side comparison confirmed that 1C2 as well as RPM.9, a MAb against rat GPV, recognized the same rat platelet molecule. In a mouse bone marrow culture, 1C2+ megakaryocytes emerged from CD41 (GPIIb)+1C2- megakaryocytes. Because 1C2+ megakaryocytes exhibited higher DNA ploidy distribution than CD41+ cells, GPV likely appears in the late stage of megakaryocyte maturation. This study established 1C2 as a MAb against mouse and rat GPV, namely CD42d, and as useful tool to study rodent megakaryopoiesis.Keywords
This publication has 23 references indexed in Scilit:
- Functional conservation of platelet glycoprotein V promoter between mouse and human megakaryocytesExperimental Hematology, 2000
- GPV Is a Marker of In Vivo Platelet Activation – Study in a Rat Thrombosis ModelThrombosis and Haemostasis, 2000
- Verotoxins induce apoptosis in human renal tubular epithelium derived cellsKidney International, 1998
- Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activationCell, 1991
- Immunocytological and Immunochemical Analysis on the Common Acute Lymphoblastic Leukemia Antigen (CALLA): Evidence that CALLA on ALL Cells and Granulocytes Are Structurally RelatedHybridoma, 1988
- SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat.Molecular and Cellular Biology, 1988
- Immunological study of in vitro maturation of human megakaryocytesBritish Journal of Haematology, 1984
- Human megakaryocytes. II. Expression of platelet proteins in early marrow megakaryocytes.The Journal of Experimental Medicine, 1981
- Human blood platelet adhesion to artery subendothelium is mediated by factor VIII–Von Willebrand factor bound to the subendotheliumNature, 1979
- Platelet plasma membrane glycoproteins Identification of a proteolytic substrate for thrombinBiochemical and Biophysical Research Communications, 1977