Response of Baboon Peripheral Blood Lymphocytes to Phytohemagglutinin: Time-Course and Dose-Response Relationships
- 1 January 1977
- journal article
- research article
- Published by S. Karger AG in Journal of Medical Primatology
- Vol. 6 (5) , 298-308
- https://doi.org/10.1159/000459761
Abstract
Optimal conditions for studying phytohemagglutinin(PHA)-induced transformation of baboon peripheral blood lymphocytes were determined. Maximal stimulation, as determined by uptake and incorporation of tritiated thymidine (total cell and trichloroacetic- acid-precipitable radioactivity), occurred at a PHA level of 12.5 μg in a culture volume of 0.25 ml containing 2 x 10^5 lymphocytes. Optimal stimulation occurred after a total incubation period of 114 h, the last 18 h of which was in the presence of 1 μCi of the labeled DNA precursor per culture. While there was considerable variation in the extent of responsiveness of lymphocytes from individual animals, the shape of the doseresponse and time-course curves for most mitogen concentrations was generally similar.Keywords
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