Conversion of pyruvic acid fermentation to tryptophan production by the combination of pyruvic acid-producing microorganisms and Enterobacter aerogenes having high tryptophanase activity.

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Abstract
Conditions for tryptophan synthesis from pyruvic acid, indole and NH4Cl by Enterobacter aerogenes AHU 1540 having a high tryptophanase activity, were investigated using a reaction mixture containing 1.7% ofpyruvic acid. Under optimum conditions, 16.4 g/liter oftryptophan was accumulated after 24 hr of incubation. Agaricus campestris AHU 9382 produced pyruvic acid in amounts of 22 - 26.5 g/liter from 5% of glucose after 3-days shaking culture. When E. aerogenes was added to this fermentation broth together with indole and NH4Cl, pyruvic acid produced was rapidly converted to tryptophan and yields of tryptophan as high as 15 g/liter were obtained after 12hr of incubation. Furthermore, pyruvic acid fermentation by Saccharomyces exiguus AHU 3110 or Corynebacterium sp. 37-3A could also be used as a pyruvic acid source for subsequent tryptophan production.

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