RELATIONSHIP BETWEEN TUMOR-FORMATION AND CELL-MEDIATED-IMMUNITY IN HAMSTERS WITH TRANSPLANTED HVJ (SENDAI VIRUS)-CARRYING TUMOR-CELLS

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 71  (3) , 325-332
Abstract
Using macrophage migration inhibition (MMI) and the cell-mediated cytotoxicity (CMC) test, the cell-mediated immune response in hamsters with transplanted HVJ-carrying tumor cells (THEL-HVJ or THEL-HVJpits) was examined in relation to the lowered transplantability of the cells. The cells cultured at a temperature (34.degree. C) permissive for HVJpits (temperature-sensitive HVJ) showed significantly lowered transplantability in hamsters. After shifting the cell culture temperature up to 39.degree. C (non-permissive for HVJpits) for 5 days, THEL-HVJpits cells which had lost their cellular HVJ antigens regained the same high transplantability observed in parent THEL cells. Culturing at 37.degree. C (partially permissive) for 24 h did not significantly affect cell tumor-forming ability with lowered transplantability, in spite of a considerable reduction in cellular HVJ antigens. The MMI test on hamsters with the transplanted tumor cells cultured at 34.degree. C (MMI/THEL-HVJ 34 or MMI/THEL-HVJpits 34) was more markedly positive, and for a longer period (1-4 wk), than the same test on hamsters inoculated with THEL cells. However, the MMI/THEL-HVJpits 39 cells acquired lowered reactivity like those from THEL-tumor bearing animals, while MMI/THEL-HVJpits 37 cells were still positive. In the CMC test, much more cytotoxic activity was observed in spleen cells from hamsters with transplanted THEL-HVJpits cells than in those from THEL-transplanted animals; there was a general correspondence with the results obtained in the above MMI test. The lowered transplantability of HVJ-carrying tumor cells may be due to a significant induction of cell-mediated immune responses. Cell membrane antigens modified (xenogenized) by the complete or partial expression of HVJ genomes carried may play an important part in this induction in vivo.