Selective cleavage of skeletal myosin subfragment‐1 to form a 26 kDa peptide which shows ATP‐sensitive actin binding
- 2 January 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 242 (2) , 275-278
- https://doi.org/10.1016/0014-5793(89)80484-3
Abstract
A 26 kDa peptide has been cleaved from the C-terminus of the S1 heavy chain with formic acid. Cleavage occurs in the ‘50 kDa’ domain probably at the Asp-600-Pro-601 bond. This fragment has been renatured in the presence of the A2 light chain and the 26 kDa(A2) complex shown to interact with actin in an ATP-sensitive manner.Keywords
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