Occurrence of long-chain fatty acids and glycolipids in the cell-envelope fractions of baker's yeast
- 1 December 1971
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 125 (4) , 963-969
- https://doi.org/10.1042/bj1250963
Abstract
1. The total yield of fatty acids from the whole envelopes was markedly higher than that obtained from the ordinary cell walls. In both samples the major fatty acids were C16 and C18 acids. 2. The whole envelopes contained C18 acids and long-chain (C19–C26) fatty acids, in a higher proportion than did the ordinary cell walls. Fifteen fatty acids with more than 18 carbon atoms were identified, among which 2-hydroxy-C26:0 and C26:0 acids predominated. 3. A complex sphingolipid containing inositol, phosphorus and mannose was isolated from the whole cell envelopes. The main fatty acids of this lipid were 2-hydroxy-C26:0 and C26:0 acids. It was concluded that this sphingolipid is present both in the ordinary cell wall and in the plasma membrane of baker's yeast. 4. The neutral lipids amounted to over 50% and the glycerophosphatides to about 30% of the total fatty acid content of the whole envelope. The major fatty acids in these lipids were C16:1, C18:1 and C16:0 acids. The proportion of fatty acids with more than 18 carbon atoms was lowest in the neutral lipids, whereas the neutral glycolipids contained the highest percentage of these fatty acids. Acidic glycolipids amounted to 14% of the total fatty acid content of the whole envelope. The presence of a cerebroside sulphate in this lipid fraction was demonstrated, whereas the high content of 2-hydroxy-C26:0 acid found is caused by the complex inositol- and mannose-containing sphingolipid.Keywords
This publication has 16 references indexed in Scilit:
- The function of very long chain fatty acids in membrane structure: Evidence from milk cerebrosidesBiochemical and Biophysical Research Communications, 1970
- The lipolytic activities of the isolated cell envelope fractions of baker's yeastBiochemical Journal, 1970
- Acylglucoses inEscherichia coli, Saccharomyces cerevisiaeandAgaricus bisporusFEBS Letters, 1970
- [Composition and distribution of lipids within cells of Saccharomyces cerevisiae].1970
- The enzymic composition of the isolated cell wall and plasma membrane of baker's yeastBiochemical Journal, 1970
- Preparation of phosphatidyl inositol from bakers' yeastJournal of Lipid Research, 1966
- Quantitative determination 0f glucose and galactose in gangliosides by gas-liquid chromatographyBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1966
- Preparation and properties of various salt forms of plant phosphatidyl inositolsLipids, 1966
- Microdetermination of lipid phosphorus as a measure of bacterial membrane substanceAnalytical Biochemistry, 1963
- A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUESJournal of Biological Chemistry, 1957