COMPARATIVE-STUDY ON GLYCOLIPID COMPOSITION BETWEEN 2 CELL-TYPES OF RAT ASCITES HEPATOMA-CELLS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 529  (1) , 96-105
Abstract
A comparative study of glycolipids was performed in 2 cell lines of rat ascites hepatomas, island-forming and free cell types, and several marked differences were found. Hexose content in glycolipids derived from AH 7974F (free cell type) was about 3.6-fold as much as that from AH 7974 (island-forming cell type) on the basis of dry cell weight. The glycolipids in AH 7974 cells were tentatively identified as glucosylceramide, lactosylceramide, galactosylgalactosylglucosylceramide, globoside and hematoside (GH3) by TLC and GLC. The glycolipids in AH 7974F cells were glucosylceramide, lactosylceramide and at least 4 unknown lipids. The structure of 1 of these unknown lipids was asialo-GM2 by methylation and enzymatic degradation studies. The presence of asialo GM1 was suggested by immunoprecipitation tests with anti-asialo-GM1 serum. Glucosylceramide and lactosylceramide from AH 7974F cells possessed hydroxy fatty acids as major fatty acids components, which were rare in these glycolipids from AH 7974 cells.