Isopyenic centrifugatian of sheared L-cell chromatin in metrizamide gradients
- 1 January 1974
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (11) , 1359-1370
- https://doi.org/10.1093/nar/1.11.1359
Abstract
Isopycnic gradient centrifugation of L-929 cell chromatin in a 38% (W/V) metrizamide solution yields two distinct fractions. The fraction banding at a density of 1.24 gm/cm(2) (H chromatin) contains about 10% of the DNA present in the fraction banding at a density of 1.18 gm/cm(2) (L chromatin). Both fractions contain the same proportions of satellite to main band DNA's. Some differences can be seen in the DNA: protein ratios and types of proteins present in the H and L chromatin fractions.Keywords
This publication has 18 references indexed in Scilit:
- Buoyant densities and hydration of nucleic acids, proteins and nucleoprotein complexes in metrizamideBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Two-dimensional electrophoresis of proteins of citric acid nuclei prepared with aid of a Tissumizer®Experimental Cell Research, 1973
- Comparison of the proteins of condensed and extended chromatin fractions of rabbit liver and calf thymusBiochemistry, 1973
- Fractionation of mouse myeloma chromatinBiochemistry, 1973
- Chromatin. Its isolation from cultured mammalian cells with particular reference to contamination by nuclear ribonucleoprotein particlesBiochemistry, 1973
- Isopycnic centrifugation of sheared chromatin in metrizamide gradientsFEBS Letters, 1973
- Deoxyribonucleic acid replication in synchronized cultured mammalian cells: I. Time of synthesis of molecules of different average guanine + cytosine contentJournal of Molecular Biology, 1970
- Chromosomal Localization of Mouse Satellite DNAScience, 1970
- Localization of mouse satellite DNA in constitutive heterochromatinExperimental Cell Research, 1970
- Studies on the intranuclear distribution and properties of mouse satellite DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968