Isolation of Stem Bromelain by Affinity Chromatography and its Partial Characterization by Gel Electrophoresis
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 2 (4) , 347-354
- https://doi.org/10.1080/00327487208065671
Abstract
Pineapple stem bromelain has been successfully isolated from an acetone powder of a crude plant extract by a single passage through a column of ∊-aminocaproyl-D-tryptophan methyl ester coupled to Sepharose 4B. If organic mercury is present during all steps of the procedure, a single band with an apparent molecular weight of 23, 000 daltons is obtained after gel electrophoresis in 1% sodium dodecyl sulfate. Upon reduction with mercaptoethanol, this single species is converted into two chains of about 15, 000 and 8, 500 daltons.Keywords
This publication has 10 references indexed in Scilit:
- Yeast hexokinase. V. Subunit structure and hybridization of isoenzymesBiochemistry, 1971
- New Forms of Bovine Carboxypeptidase B and Their Homologous Relationships to Carboxypeptidase AProceedings of the National Academy of Sciences, 1971
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- The melecular weight of the undegraded polypeptide chain of yeast hexokinaseBiochemical and Biophysical Research Communications, 1970
- [18] Bromelain enzymesPublished by Elsevier ,1970
- Selective enzyme purification by affinity chromatography.Proceedings of the National Academy of Sciences, 1968
- On the Molecular Weights of the Proteolytic Enzymes of Stem Bromelain*Biochemistry, 1964
- Preparation and Chemical Properties of Purified Stem and Fruit Bromelains*Biochemistry, 1964
- Purification and Physical Characterization of Stem Bromelain*Biochemistry, 1964
- Determination of Protein-Bound Carbohydrate in Serum by Modified Anthrone MethodAnalytical Chemistry, 1960