ANTIBODY SYNTHESIS IN VITRO. XII. ACTIVITY OF RECONSTITUTED AND HYBRID RECOMBINED RIBOSOMES IN CELL FREE SYNTHESIS OF ANTIBODY*

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Abstract
SUMMARY: In a cell free system derived from peripheral human leucocytes an informative RNA (i‐RNA) was synthesized after antigenic stimulation with benzoyl‐penicilloyl‐formyl‐l‐lysine (BPoFLys). This i‐RNA was used to synthesize antibody in a second cell free system. The ribosomes used in the antibody synthesizing system were derived from peripheral human leucocytes, from human lymphoblasts CCRF and from HeLa cells. Furthermore, hybrid recombined ribosomes from 60S HeLa‐subunits and 45 S CCRF‐subunits as well as from 45S HeLa‐ and 60S CCRF‐subunits were used to synthesize antibody. Only ribosomes from peripheral human leucocytes, from CCRF‐cells and hybrid recombined ribosomes from 60S HeLa‐ and 45S CCRF‐subunits were able to synthesize antibody in the cell free system. Antibody synthesized in the cell free system was detected by phage neutralization test and by equilibrium dialysis. By these methods new markers could be demonstrated which are essential for the differentiation to an antibody synthesizing cell.