The isolation of pregnane-3(α)-ol-20-one from the hydrolysis products of ‘sodium pregnanediol glucuronidate’

Publisher Website
Google Scholar
Abstract
Pregnane-3(a)-ol-20-one was isolated from HC1 hydrolysis products of sodium pregnanediol glucuronidate prepared from human pregnancy urine. Na pregnanediol glucuronidate purified by precipitation from aqueous acetone with dry acetone and by re-crystallization from 90% (v/v) aqueous ethanol, contains about 20% of a water soluble derivative of pregnane-3(a)-ol-20-one, which could not be removed by further purification using the same methods. This probably accounts for failure to obtain more than 70% of the theoretic yield of pregnanediol upon acid hydrolysis.