Inducible nitric oxide synthase (iNOS) activity promotes ischaemic skin flap survival
Open Access
- 1 April 2001
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 132 (8) , 1631-1638
- https://doi.org/10.1038/sj.bjp.0703944
Abstract
We have examined the role of nitric oxide (NO) in a model of functional angiogenesis in which survival of a skin flap depends entirely on angiogenesis to provide an arterial blood supply to maintain tissue viability. The different effects of nitric oxide synthase (NOS) inhibitors on rat skin flap survival appeared to be explained on the basis of their NOS isoform selectivity. Skin flap survival was decreased by iNOS‐selective (inducible NOS) inhibitors, S‐methyl‐isothiourea, aminoguanidine and aminoethylthiorea; unaffected by the non‐selective inhibitor nitro‐imino‐L‐ornithine; and enhanced by the cNOS (constitutive NOS, that is endothelial NOS (eNOS) and neuronal NOS (nNOS)) inhibitor, nitro‐L‐arginine methyl ester. Skin flap survival was reduced in mice with targeted disruption of the iNOS gene (iNOS knockout mice), and the administration of nitro‐L‐arginine methyl ester significantly increased flap survival in iNOS knockout mice (PBritish Journal of Pharmacology (2001) 132, 1631–1638; doi:10.1038/sj.bjp.0703944Keywords
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