Unfixed Material Embedded in A Methacrylate Resin (Technovit 7100) For Immunofluorescent Staining
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 62 (2) , 73-75
- https://doi.org/10.3109/10520298709107971
Abstract
Wheat grains were embedded with or without fixation in Technovit 7100 or in paraffin. This enabled us to produce 4 μm sections for imnwnofluorescent staining to see whether serum of patients with baker's asthma contained IgG antibodies against wheat grains. Embedding without fixation in Technovit 7100 appeared to be suitable for unmunofluorescent staining and gave superior results to protocols requiring fixation.This publication has 2 references indexed in Scilit:
- SOME CHARACTERISTICS OF IMMUNOFLUORESCENCE TESTS FOR ANTIBODIES AGAINST GLUTEN, USING WHEAT GRAIN SECTIONS OR GLIADIN COATED SEPHAROSE BEADSActa Pathologica Microbiologica Scandinavica Section C Immunology, 1981
- Wheat grains: A substrate for the determination of gluten antibodies in serum of gluten-sensitive patientsJournal of Immunological Methods, 1977