Sequencing and the alignment of structural genes in the nqr operon encoding the Na+‐translocating NADH‐quinone reductase from Vibrio alginolyticus
- 17 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 363 (1-2) , 75-77
- https://doi.org/10.1016/0014-5793(95)00283-f
Abstract
We previously cloned a part of nqr operon encoding the Na+‐translocating NADH‐quinone reductase (NQR) from the marine Vibrio alginolyticus (Hayashi et al., FEBS Lett. 356 (1994) 330–332]. From its nucleotide sequences, four consecutive open reading frames (ORF) encoding the γ‐subunit (27.7 kDa), two unidentified ORFs of 22.6 kDa and 21.5 kDa, and the β‐subunit (45.3 kDa) were recognized. The gene encoding the α‐subunit was located upstream, and together with the recent report by Beattie et al. [FEBS Lett. 356 (1994) 333–338], the nqr operon was found to be constructed from six consecutive structural genes, where nqrl, nqr3 and nqr6 correspond to the α‐, γ‐, and β‐subunits, respectively, of the NQR complex.Keywords
This publication has 13 references indexed in Scilit:
- Cloning and sequencing of four structural genes for the Na+‐translocating NADH‐ubiquinone oxidoreductase of Vibrio alginolyticusFEBS Letters, 1994
- Cloning of the Na+‐translocating NADH‐quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the β‐subunit in Escherichia coliFEBS Letters, 1994
- Correlation between the respiration-driven Na+ pump and Na+-dependent amino acid transport in moderately halophilic bacteriaJournal of General Microbiology, 1993
- The Gene Locus of the Proton-translocating NADH : Ubiquinone Oxidoreductase in Escherichia coliJournal of Molecular Biology, 1993
- Primary structures of two subunits of NADH:ubiquinone reductase from Neurospora crassa concerned with NADH-oxidation. Relationship to a soluble NAD-reducing hydrogenase of Alcaligenes eutrophusBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1991
- The NADH-binding subunit of the energy-transducing NADH-ubiquinone oxidoreductase of Paracoccus denitrificans: gene cloning and deduced primary structureBiochemistry, 1991
- Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenaseBiochemistry, 1991
- Sodium-transport NADH-quinone reductase of a marineVibrio alginolyticusJournal of Bioenergetics and Biomembranes, 1989
- Subunit component and their roles in the sodium-transport NADH: quinone reductase of a marine bacterium, Vibrio alginolyticusBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1987
- Characterization of the Na+-dependent respiratory chain NADH:quinone oxidoreductase of the marine bacterium, Vibrio alginolyticus, in relation to the primary Na+ pumpBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1984