Hippuric acid synthesis in man after the administration of [α-14C]glycine

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Abstract
The specific activity of the glycine conjugated with benzoate was determined in 3 persons after administration of labeled glycine. Rate of decrease of specific activity of the hippuric acid can be described in terms of 2 exponential components having average half-times of 6.8 and 75 days. Rates for these 2 components agree well with rates of the 2 slowest components of C14O2 excretion in the breath. The observed specific activity of the hippuric acid was almost entirely confined to the alpha-carbon atom of the glycine moiety except during the period 85-106 days after administration. The tail portion of the curve of the specific activity of hemin or hemoglobin could be due largely, if not entirely, to the continual formation of red cells containing C14 derived from persisting labeled glycine, and is probably not due to a population of red cells having life spans far in excess of 120 days.