Ultrastructure of multilayered cultures of human gingival epithelial cells: attachment to enamel surfaces in vitro

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Abstract
Human gingival cells were collected using an enzymatic procedure and seeded on a feeder layer of irradiated mouse 3T3 fibroblasts. Epithelial cells generate stratified colonies ultimately forming an epithelium which was studied using electron microscopy. When this epithelium‐like structure was dispased and transferred to enamel surfaces, the relationship between basal cells and enamel corresponded to half‐desmosomes and a discontinuous extracellular matrix.