Thermodynamic Analysis of Autonomous Parallel β-Sheet Formation in Water
- 12 May 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (22) , 7148-7149
- https://doi.org/10.1021/ja060942p
Abstract
We report the first thermodynamic analysis of parallel β-sheet formation in a model system that folds in aqueous solution. NMR chemical shifts were used to determine β-sheet population, and van't Hoff anaysis provided thermodynamic parameters. Our approach relies upon the d-prolyl-1,1-dimethyl-1,2-diaminoethane unit to promote parallel β-sheet formation between attached peptide strands. The development of a macrocyclic reference molecule to provide chemical shift data for the fully folded state was crucial to the quantitative anaylsis.Keywords
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