Affinity Chromatography of Human Leukocyte and Diploid Cell Interferons on Sepharose-Bound Antibodies
- 1 February 1975
- journal article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 114 (2_Part_1) , 640-644
- https://doi.org/10.4049/jimmunol.114.2_part_1.640
Abstract
Interferons produced in human peripheral leukocytes (LE) and foreskin fibroblast (FS-4) cells were subjected to affinity chromatography on Sepharose-bound globulins from rabbits immunized with these interferons. Anti-LE interferon sera neutralized both interferons, but titers against FS-4 interferon were consistently lower than those against LE interferon. Anti-FS-4 interferon sera neutralized only FS-4 but not LE interferon. Accordingly, affinity columns constructed with anti-FS-4 globulin excluded LE but not FS-4 interferon, whereas those prepared with anti-LE interferon globulin bound and eluted both LE and FS-4 interferons. Purification of native interferons of both types on anti-LE interferon-Sepharose ranged from 680- to 3,600-fold and recoveries from 72 to 126%. Specific activities of eluate pools varied from 4 to 30 × 106 reference (B, 69/19) units per milligram protein.This publication has 0 references indexed in Scilit: