fbpABC Gene Cluster in Neisseria meningitidis Is Transcribed as an Operon
- 1 December 2000
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 68 (12) , 7166-71
- https://doi.org/10.1128/iai.68.12.7166-7171.2000
Abstract
The neisserial fbpABC locus has been proposed to constitute a single transcriptional unit. To confirm this operonic arrangement, transcription assays using reverse transcriptase PCR amplification were conducted with Neisseria meningitidis . The presence of fbpAB and fbpBC transcripts obtained by priming cDNA synthesis with an fbpC -sequence-specific oligonucleotide indicates that fbpABC is organized as a single expression unit. The ratio of fbpA to fbpABC mRNA was approximately between 10- to 20-fold, as determined by real-time quantitative PCR.Keywords
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