Plasmid pT181 replication is regulated by two countertranscripts.
- 1 February 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (3) , 638-642
- https://doi.org/10.1073/pnas.82.3.638
Abstract
A transcription map of the replication control region of the Staphylococcus aureus plasmid pT181 was constructed. Two major leftward transcripts, RNA III and RNA IV, start at positions 339 and 413, respectively. These 2 RNA can serve as mRNA for a plasmid-specific replication protein RepC. Two short rightward transcripts, RNA I and RNA II, .apprxeq.85 and 150 nucleotides long, respectively, start at position 246. These rightward transcripts (referred to as countertranscripts) do not appear to be translated but act directly as negative regulators of plasmid replication, probably by interfering with translation of the RepC mRNA. There is no significant base sequence homology among the countertranscripts of pT181, ColE1 and R1/NR1/R6-5, suggesting that the structural parallelism has risen by convergent molecular evolution.This publication has 27 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Control of replication of bacterial plasmids: Genetics, molecular biology, and physiology of the plasmid R1 systemPlasmid, 1984
- Base pairing of RNA I with its complementary sequence in the primer precursor inhibits ColE1 replicationNature, 1981
- Replication of plasmid pT181 DNA in vitro: requirement for a plasmid-encoded product.Proceedings of the National Academy of Sciences, 1981
- Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA.Proceedings of the National Academy of Sciences, 1981
- Optimal computer folding of large RNA sequences using thermodynamics and auxiliary informationNucleic Acids Research, 1981
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinatesNucleic Acids Research, 1979