Structure and Function of the Genome of Coliphage T5
- 3 March 1973
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 33 (3) , 517-528
- https://doi.org/10.1111/j.1432-1033.1973.tb02711.x
Abstract
Separated strands of ligated DNA from coliphage T5+ and single‐stranded fragments purified from alkaline‐denatured DNA were used in DNA‐DNA annealing experiments. The products of such experiments were analyzed by analytical ultracentrifugation to equilibrium in a CsCl density gradient as well as by electron microscopy. Pycnometric analyses showed that the heavy strand is the uninterrupted strand, whereas the light strand is the ligated form of the nicked strand. All fragments of length shorter than one half of the complete DNA molecule are derived from the light strand. A new arrangement of the single‐stranded fragments of the T5+ chromosome has been deduced from electron microscopic analysis and has resulted in a new model of the physical structure of the DNA of T5+.This publication has 20 references indexed in Scilit:
- The chromosome of bacteriophage T5: I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresisJournal of Molecular Biology, 1972
- The chromosome of bacteriophage T5: II. Arrangement of the single-stranded DNA fragments in the T5+ and T5st(O) chromosomesJournal of Molecular Biology, 1972
- Genetic and physiological studies of bacteriophage T5 2. The relationship between phage DNA synthesis and protein synthesis in T5-infected cellsBiochemical and Biophysical Research Communications, 1971
- Molecular weights of coliphages and coliphage DNA: III. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from bovine papilloma virusJournal of Molecular Biology, 1970
- Electron microscopy of single-stranded DNAJournal of Molecular Biology, 1970
- Analysis of the interruptions in bacteriophage T5 DNAJournal of Molecular Biology, 1970
- Electron microscopy of size and shape of viral DNA in solutions of different ionic strengthsJournal of Molecular Biology, 1967
- The anatomy of the T5 bacteriophage DNA moleculeJournal of Molecular Biology, 1966
- Molecular aspects of DNA transfer from phage T5 to Host CellsJournal of Molecular Biology, 1964
- Molecular aspects of DNA transfer from phage T5 to host cellsJournal of Molecular Biology, 1964