Stilbene Synthase and Chalcone Synthase

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Abstract
Cultured cells of P. excelsa capable of forming stilbenes and flavanoids meet established. Unlike needles of intact plants containing piceatannol (3,3'',4'',5-tetrahydroxystilbene) and stilbene glycosides the cultured cells converted phenylalanine and p-coumaric acid primarily into resveratrol monomethyl ether (3,4''-dihydroxy-5-methoxystilbene) and naringenin. Partially purified enzyme preparations were assayed for chalcone synthase and for stilbene synthase activity converting malonyl-CoA plus p-coumaroyl-CoA into 3,4''-5-trihydroxystilbene (resveratrol). Although stilbene synthase and chalcone synthase use the same substrates and exhibit similar molecular properties, i.e., MW and subunit MW, they are 2 different proteins. This difference was demonstrated by gel electrophoresis and by means of monospecific antibodies.