Genetic Typing of Shiga Toxin 2 Variants ofEscherichia coliby PCR-Restriction Fragment Length Polymorphism Analysis
Open Access
- 1 October 2004
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 70 (10) , 6309-6314
- https://doi.org/10.1128/aem.70.10.6309-6314.2004
Abstract
Shiga toxins Stx1 and Stx2 play a prominent role in the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections. Several variants of the stx2 gene, encoding Stx2, have been described. In this study, we developed a PCR-restriction fragment length polymorphism system for typing stx2 genes of STEC strains. The typing system discriminates eight described variants and allows the identification of new stx2 variants and STEC isolates carrying multiple stx2 genes. A phylogenetic tree, based on the nucleotide sequences of the toxin-encoding genes, demonstrates that stx2 sequences with the same PvuII HaeIII HincII AccI type generally cluster together.Keywords
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