Photoreduction and Photooxidation of Cytochrome c by Spinach Chloroplast Preparations.

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Abstract
This paper describes the behavior toward cytochrome c of spinach chloroplasts prepared in a suspending medium of 0.35 [image] NaCl. The chloroplasts cause a photoreduction of ferricytochrome c, and a dark oxidation of ferrocytochrome c. They also contain a cytochrome c photooxidase, which can be clearly demonstrated after the photoreduction reaction has been inhibited with digitonin. The enzyme or enzyme complex causing the photooxidation of cytochrome c can be solubilized by treatment with digitonin. The cytochrome c photooxidase is not inhibited by cyanide and may thus be distinguished from the cyanide-sensitive dark oxidase.