In Vitro Synthesis of Procollagen on Polysomes
- 1 September 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (9) , 2727-2731
- https://doi.org/10.1073/pnas.69.9.2727
Abstract
The major collagenous products synthesized in a cell-free polysome preparation are pro-α1 and pro-α2, formed in a ratio of 2:1. They are the precursor forms of α1 and α2 chains of normal connective-tissue collagen that are also formed in small amounts. A procollagen peptidase activity has been demonstrated in the supernatant fraction that can account for the formation of α1 and α2 chains from their precursors. The polysomal system is activated by a salt extract of reticulocyte ribosomes and is inhibited by aurintricarboxylic acid, suggesting that the polysomes are able to initiate protein synthesis.Keywords
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