Complexity and specificity of polysomal poly(A+) RNA in mouse tissues

Publisher Website
Google Scholar
Abstract
Base sequence complexities of polysomal poly(A+) RNA from mouse embryo, brain and liver were estimated by hybridization to homologous cDNA [complementary DNA] to be approximately 7 .times. 109, 1.5 .times. 1010 and 7 .times. 109 daltons, respectively. By annealing each cDNA with a large excess of total mouse embryo DNA, the genes coding for the polysomal poly(A+) sequences were unique. Heterologous hybridization experiments showed that the high abundance class of poly(A+) sequences in one tissue is not identical with the high abundance class in other tissues. At least 55%, and possibly more, of the poly(A+) RNA in one tissue is present in the poly(A+) RNA of another tissue.

This publication has 3 references indexed in Scilit: