siRNA Cell Arrays for High-Content Screening Microscopy
Open Access
- 1 September 2004
- journal article
- research article
- Published by Taylor & Francis in BioTechniques
- Vol. 37 (3) , 454-462
- https://doi.org/10.2144/04373rt01
Abstract
RNA interference (RNAi) is a recent advance that provides the possibility to reduce the expression of specific target genes in cultured mammalian cells with potential applications on a genome-wide ... siRNA Cell Arrays RNA interference (RNAi) is emerging as a powerful tool for genome-wide functional screening of proteins. Robust methods are needed, therefore, to implement this approach in a high...Keywords
Funding Information
- Federal Ministry of Education and Research (01GR0101, 01KW0013)
- Verbundforschung Baden-Wuerttemberg (24-720.431-1-2/2)
This publication has 13 references indexed in Scilit:
- A microscope‐based screening platform for large‐scale functional protein analysis in intact cellsFEBS Letters, 2003
- High-Throughput Selection of Effective RNAi Probes for Gene SilencingGenome Research, 2003
- RNAi Microarray Analysis in Cultured Mammalian CellsGenome Research, 2003
- Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cellsNature, 2001
- Microarrays of cells expressing defined cDNAsNature, 2001
- Systematic subcellular localization of novel proteins identified by large‐scale cDNA sequencingEMBO Reports, 2000
- Sec31 encodes an essential component of the COPII coat required for transport vesicle budding from the endoplasmic reticulum.Molecular Biology of the Cell, 1997
- Characterization of a cis-Golgi matrix protein, GM130.The Journal of cell biology, 1995
- Disruptions in Golgi structure and membrane traffic in a conditional lethal mammalian cell mutant are corrected by epsilon-COP.The Journal of cell biology, 1994
- β-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivoPublished by Elsevier ,1993