Neuroprotection and neurorescue against Aβ toxicity and PKC‐dependent release of non‐amyloidogenic soluble precursor protein by green tea polyphenol (‐)‐epigallocatechin‐3‐gallate
- 28 March 2003
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 17 (8) , 1-23
- https://doi.org/10.1096/fj.02-0881fje
Abstract
SPECIFIC AIMSWe recently demonstrated that the neuroprotective mechanism of (-)-epigallocatechin-3-gallate (EGCG) against oxidative stress (OS)-induced cell death includes stimulation of protein kinase C (PKC) and modulation of cell survival/cell cycle genes in human SH-SY5Y neuroblastoma cells. Considering these findings, we examined in the present study: the 1) neuroprotective and neurorescue effects of EGCG on β amyloidpeptide (Aβ)-induced neurotoxicity, 2) regulation of amyloid precursor protein-α (APP-α) processing, and 3) involvement of PKC-dependent mechanisms in the modulation of soluble APP-α (sAPPα) release in cell culture and in vivo.PRINCIPAL FINDINGS1. Stimulation of sAPPα release from SH-SY5Y and PC12 cells by EGCGTreatment of human SH-SY5Y neuroblastoma cells for 2 h with increasing concentrations of EGCG resulted in a concentration-dependent increase in sAPPα released into the conditioned media. Stimulation occurred with doses as low as 1 μM EGCG; maximal stimulation was obtained at 10 μM,...Keywords
Funding Information
- National Parkinson Foundation
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