A dsbA mutant of Pseudomonas syringae exhibits reduced virulence and partial impairment of type III secretion
- 1 March 2000
- journal article
- research article
- Published by Wiley in Molecular Plant Pathology
- Vol. 1 (2) , 139-150
- https://doi.org/10.1046/j.1364-3703.2000.00016.x
Abstract
To identify virulence genes of P. syringae pv. tomato strain DC3000 we screened for mutants with reduced virulence on its plant hosts, Arabidopsis thaliana and tomato. We isolated a Tn5‐insertion mutant that exhibited reduced virulence on both hosts. Further characterization showed that this mutant carried a single Tn5 insertion in the dsbA gene, which encodes a periplasmic disulphide bond‐forming protein. In addition to reduced virulence, the dsbA mutant exhibits mucoid colony morphology, loss of fluorescence, decreased motility, and a reduced growth rate in culture. The dsbA mutant is able to multiply in A. thaliana and tomato plants, trigger the hypersensitive response on tobacco and elicit Pto‐mediated resistance in tomato, indicating that type III secretion occurs in this background. However, type III secretion appears to function with reduced efficiency in the dsbA mutant, as type III‐dependent secretion of HrpZ and AvrRpt2 is impaired. These findings indicate that while the dsbA gene is required for multiple cellular functions in P. syringae, type III secretion in P. syringae is only partially dependent on dsbA.Keywords
This publication has 62 references indexed in Scilit:
- The Rieske protein from Paracoccus denitrificans is inserted into the cytoplasmic membrane by the twin‐arginine translocaseThe FEBS Journal, 2006
- Secretion of YscP from Yersinia enterocolitica is essential to control the length of the injectisome needle but not to change the type III secretion substrate specificityMolecular Microbiology, 2005
- Evidence That the Pseudomonas syringae pv. syringae hrp-Linked hrmA Gene Encodes an Avr-Like Protein That Acts in an hrp-Dependent Manner Within Tobacco CellsMolecular Plant-Microbe Interactions®, 1997
- Structure of TcpG, the DsbA protein folding catalyst from Vibrio choleraeJournal of Molecular Biology, 1997
- Differential effect of dsbA and dsbC mutations on extracellular enzyme secretion in Erwinia chrysanthemiMolecular Microbiology, 1995
- TheavrRpm1Gene ofPseudomonas syringaepv.maculicolaIs Required for Virulence on ArabidopsisMolecular Plant-Microbe Interactions®, 1995
- RPS2, an Arabidopsis disease resistance locus specifying recognition of Pseudomonas syringae strains expressing the avirulence gene avrRpt2.Plant Cell, 1993
- ArabidopsisMutations at theRPS2Locus Result in Loss of Resistance toPseudomonas syringaeStrains Expressing the Avirulence GeneavrRpt2Molecular Plant-Microbe Interactions®, 1993
- Identification of a protein required for disulfide bond formation in vivoPublished by Elsevier ,1991
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983