Horseradish-Root Tissue Tubular Reactor for Flow Injection Detection of Hydrogen Peroxide

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Abstract
The use of a horseradish-root tissue as a on-line reactor in connection with flow injection measurements of hydrogen peroxide is described. Such tissue reactor is very suitable for flow operation, as it couples the advantages of very high enzyme loading and good durability, with extreme simplicity and economy. The high conversion efficiency is evaluated under various experimental conditions (reactor dimensions, flow rate, etc.). Response characteristics, such as linearity, reproducibility or response time, are reported. The incorporation of glucose oxidase in the carrier solution allows convenient, low potential, monitoring of glucose (through the liberation and conversion of hydrogen peroxide). Analogous measurements of dopamine, based on an open-tubular apple reactor, are reported. Tissue biocatalytic reactors thus offer a very attractive alternative to enzyme reactors used for flow analysis.