Effect of Neomycin and Protein S1 on the Binding of Streptomycin to the Ribosome

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Abstract
The binding of [3H]dihydrostreptomycin to the [Escherichia coli] 70-S ribosome or the 30-S subunit was investigated in the presence of neomycin by the Millipore filtration or the equilibrium dialysis procedure. Dihydrostreptomycin binds equally well to the 30-S subunit and the 70-S ribosome, and neomycin stimulates the binding of dihydrostreptomycin to the ribosome by increasing the association constant and not by creating new binding sites. Specific removal of protein S1 from the 30-S subunit neither affected the binding of dihydrostreptomycin to the ribosome nor the stimulation of dihydrostretomycin binding by neomycin.