CHEMICAL COMPOSITION OF LIPOPOLYSACCHARIDE FROM PSEUDOMONAS-AERUGINOSA STRAIN PAO AND A SPONTANEOUSLY DERIVED ROUGH MUTANT

  • 1 January 1977
    • journal article
    • research article
    • Vol. 19  (76) , 103-116
Abstract
The chemical composition of the lipopolysaccharide (LPS) of the smooth strain P. aeruginosa PAO 307 and a spontaneously derived rough mutant, obtained by selection for resistance to the LPS-specific phage E79, are compared. The rough LPS contained lipid A, heptose, 2-keto-3-deoxyoctonic acid, galactosamine, alanine and phosphate but lacked glucose, rhamnose and fucosamine which were important constituents, on a weight basis, of the smooth LPS. These results and chromatographic analysis of the polysaccharide fraction indicate that the rough strain lacked side chain material and was defective in its inner core region. The chemical date obtained were consistent with a core in the PAO strain similar to that of strain NCTC 1999, enhancing the evidence for a common core polysaccharide in the LPS of P. aeruginosa strains.