Rbx1, a Component of the VHL Tumor Suppressor Complex and SCF Ubiquitin Ligase
- 23 April 1999
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 284 (5414) , 657-661
- https://doi.org/10.1126/science.284.5414.657
Abstract
The von Hippel–Lindau (VHL) tumor suppressor gene is mutated in most human kidney cancers. The VHL protein is part of a complex that includes Elongin B, Elongin C, and Cullin-2, proteins associated with transcriptional elongation and ubiquitination. Here it is shown that the endogenous VHL complex in rat liver also includes Rbx1, an evolutionarily conserved protein that contains a RING-H2 fingerlike motif and that interacts with Cullins. The yeast homolog of Rbx1 is a subunit and potent activator of the Cdc53-containing SCF Cdc4 ubiquitin ligase required for ubiquitination of the cyclin-dependent kinase inhibitor Sic1 and for the G 1 to S cell cycle transition. These findings provide a further link between VHL and the cellular ubiquitination machinery.Keywords
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