Degradation of NAD(H) by Endogenous Enzymes of Yeasts and Clostridia
Open Access
- 1 February 1986
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 41 (1-2) , 172-178
- https://doi.org/10.1515/znc-1986-1-226
Abstract
The time course of degradation of exogenous NAD and NADH (2.5 mM) catalyzed by endogenous enzymes present in Saccharomyces cerevisiae, Candida utilis, Clostridum sp. La 1, Clostridium kluyveri, and Clostridium sporogenes have been determined. The half lives of the pyridine nucleotides depend extremely on the organism and, for the same organism, on the growth conditions. C. sp. La 1 as well as C. kluyveri possess only negligible enzyme activities for NAD degradation. However, C. sporogenes shows activities leading to half lives of less than 2 h for NAD and 5 h for NADH. At 25.degree. C half lives in the order of 5-17 h have been observed for Candida utilis under different conditions. The half lives of NAD are roughly 5 times higher in the presence of Saccharomyces cerevisiae.Keywords
This publication has 4 references indexed in Scilit:
- Unconventional and effective methods for the regeneration of NAD(P) H in microorganisms or crude extracts of cellsJournal of Biotechnology, 1984
- On the occurrence of enoate reductase and 2-oxo-carboxylate reductase in clostridia and some observations on the amino acid fermentation by Peptostreptococcus anaerobiusArchiv für Mikrobiologie, 1983
- Enzyme-catalyzed organic synthesis: NAD(P)H cofactor regeneration by using glucose-6-phosphate and the glucose-5-phosphate dehydrogenase from Leuconostoc mesenteroidesJournal of the American Chemical Society, 1981
- The activities of hydrogenase and enoate reductase in two Clostridium species, their interrelationship and dependence on growth conditionsArchiv für Mikrobiologie, 1980